Journal article
Cu(ATSM) Increases P-Glycoprotein Expression and Function at the Blood-Brain Barrier in C57BL6/J Mice
J Pyun, HJ Koay, P Runwal, C Mawal, AI Bush, Y Pan, PS Donnelly, JL Short, JA Nicolazzo
Pharmaceutics | MDPI | Published : 2023
Abstract
P-glycoprotein (P-gp), expressed at the blood-brain barrier (BBB), is critical in preventing brain access to substrate drugs and effluxing amyloid beta (Aβ), a contributor to Alzheimer’s disease (AD). Strategies to regulate P-gp expression therefore may impact central nervous system (CNS) drug delivery and brain Aβ levels. As we have demonstrated that the copper complex copper diacetyl bis(4-methyl-3-thiosemicarbazone) (Cu(ATSM)) increases P-gp expression and function in human brain endothelial cells, the present study assessed the impact of Cu(ATSM) on expression and function of P-gp in mouse brain endothelial cells (mBECs) and capillaries in vivo, as well as in peripheral organs. Isolated ..
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Awarded by University of Melbourne
Funding Acknowledgements
Jae Pyun was supported by the Australian Government Research Training Program Scholarship. This study was also supported in part by funding from the Australian Research Council(DP200100178) and the National Health and Medical Research Council (APP2011853). The Australian Cancer Research Foundation provided support for the NMR spectrometer used in this research(Bio21). We thank the Mass Spectrometry and Proteomics Facility at Bio21 Institute, University of Melbourne. Professor Ashley I. Bush is supported by a National Health and Medical Research Council L3 Investigator Grant (1194028). Open access publishing facilitated by Monash University, as part of the MDPI-Monash University agreement via the Council of Australian University Librarians.